Optimization of acid hydrolysis conditions of delimed tannery fleshings by response surface method
Year: 2009
Type: article
Abstract: An ecofriendly method, involving mild organic acids to hydrolyse delimed tannery fleshings (TF), has been developed. Combination of formic acid and propionic acid (1:1) was employed to obtain hydrolysates with higher degree of hydrolysis (DH). Better antioxidant properties were obtained by response surface method (RSM) using a factorial design. Effect of pretreatment involving steam cooking (80°C for 15 min) indicated that cooked delimed TF on acid treatment had significantly (P0.5) higher protein extractability than uncooked delimed TF. Optimized levels of factors for obtaining highest DH (51%) at room temperature for cooked delimed TF were acid mixture (20%) and incubation time (9 days). Liquid hydrolysate from TFs exhibited antioxidant activity (125.56 mg ascorbic acid equivalents /ml extract) and diphenyl picrylhydrazyl (DPPH) radical scavanging activity (46.42%). Chemical score of hydrolysate revealed an excess amount of essential amino acids (arginine, leucine and lysine) as compared to reference protein. This indicates its potential for use in livestock/aquaculture feeds
Source: CFTRI Institutional Repository
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2291130791