Effects of porcine jelly matrix (JMX) on gene expression of porcine umbilical cord (PUC) stem cells
Year: 2014
Type: dissertation
Abstract: Culturing stem cells is usually done on tissue-culture treated plastic.Over time the cells change their gene expression and start to differentiate.Porcine umbilical cord (PUC) stem cells express the embryonic transcription factors Oct4, Nanog and Sox2 and changes in their expression may be useful for to evaluating culture-induced changes in the cells.We developed an extract of porcine Wharton's jelly matrix (JMX) that may provide some characteristics of the stem cell niche located in the umbilical cord.Our extract used whole cords and enzyme digestion to simplify preparation of the product.We compare cells cultured on Table of Contents Table of Contents ........
Author: Jodi M Morton
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2291330060