Work

Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity

Year: 2003

Type: book-chapter

Abstract: Numerous growth factors, hormones, and transforming oncogenes activate phosphatidylinositol 3-kinase (PI3K), an enzyme that phosphorylates the 3′-hydroxyl position of phosphoinositides to generate the intracellular messengers phosphatidylinositol-3-phosphate [PI(3)P], phosphatidylinositol-3,4-bisphos-phate [PI(3,4)P 2 ], and phosphatidylinositol-3,4,5-trisphosphate [PI(3,4,5)P 3 ] (reviewed in ref. 1 ). PI3K commonly participates in signaling pathways leading to increased cell survival, proliferation, and growth, but in some cells and tissues it regulates more specialized biological processes. Notably, PI3K is an obligate intermediate in the signaling pathway linking insulin’s arrival at the cell surface to the regulation of glucose uptake and anabolic metabolism (reviewed in ref. 2 ). This chapter describes a method for quantifying the activation of the class 1A type PI3Ks by insulin. These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Source: Diabetes Mellitus

DOI: 10.1385/1-59259-377-1:127

Authors: Li-Ping Wang, Scott A. Summers

Institution: Colorado State University

Language: English

Topic: PI3K/AKT/mTOR signaling in cancer

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: diamond

Funders: American Diabetes Association, American Heart Association, University of Pennsylvania, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: The role of sphingolipids in insulin signal transduction, G4418727129