Work

Combinatorial Generation of Chemical Diversity by Redox Enzymes in Chaetoviridin Biosynthesis

Year: 2016

Type: article

Abstract: Chaetoviridins constitute a large family of structurally related secondary metabolites isolated from Chaetomium fungi. To elucidate the biosynthesis pathway and understand how the chemical diversity of chaetoviridins is generated, gene deletion and in vitro characterization of the four post-PKS modifications enzymes were undertaken. CazL and CazP were identified to have substrate promiscuity that facilitates the formation of nonchlorinated analogues. In addition, enzymatic oxidation and reduction combined with spontaneous dehydration and lactonization of the intermediates further expand the chemical diversity.

Source: Organic Letters

DOI: 10.1021/acs.orglett.6b00380

Authors: Michio Sato, Jaclyn M. Winter, Shinji Kishimoto, Hiroshi Noguchi, Yi Tang, Kenji Watanabe

Institutions: Shizuoka University, University of Shizuoka, University of California, Los Angeles

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

Open Access status: green