Functional cloning of a gp100‐reactive T‐cell receptor from vitiligo patient skin
Year: 2016
Type: article
Abstract: We isolated gp100-reactive T cells from perilesional skin of a patient with progressive vitiligo with superior reactivity toward melanoma cells compared with tumor-infiltrating lymphocytes 1520, a melanoma-derived T-cell line reactive with the same cognate peptide. After dimer enrichment and limited dilution cloning, amplified cells were subjected to reverse transcription and 5' RACE to identify the variable TCRα and TCRβ subunit sequences. The full-length sequence was cloned into a retroviral vector separating both subunits by a P2A slippage sequence and introduced into Jurkat cells and primary T cells. Cytokine secreted by transduced cells in response to cognate peptide and gp100-expressing targets signifies that we have successfully cloned a gp100-reactive T-cell receptor from actively depigmenting skin.
Source: Pigment Cell & Melanoma Research
DOI: 10.1111/pcmr.12458
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Awards: The interaction of IDO and Tregs Leads to Immunosuppression in Glioma, Autoimmune vitiligo as a roadmap to melanoma therapy, G2138925677, The interaction of IDO and Tregs Leads to Immunosuppression in Glioma, G5772555201, TCR Gene Modified T Cells for Adoptive Immunotherapy, G8182147526, Separating autoimmunity and anti-tumor immunity
OpenAlex ID: W2296183003