Determinants of infection in the peritoneal cavity. III. The action of selected inhibitors on the fate of Staphylococcus aureus in the mouse.
Year: 1962
Type: article
Abstract: Staphylococcus aurous in the Mouse** The preceding paper presented data illustrating the importance of the number of phagocytes on the fate of S. aureus in the peritoneal cavity of the mouse. These findings, in many instances, took place under conditions in which there was no major change in the functional activity of the individual mononuclear or polymorphonuclear leucocyte. Previous studies from this laboratory have demonstrated in vitro that inhibitors of glycolysis interfere with the phagocytic activity of the rabbit peritoneal exudate granulocyte. Certain of these agents, i.e., arsenite, have been employed in vivo by Berry and Mitchell ' and diminish the survival time of mice infected intraperitoneally with Salmonella typhimurium. Other inhibitors and intermediates, e.g., malonate, citrate and succinate, were also studied by the same group,1'3 and it was suggested that they interfered with the normal functioning of the Krebs, tricarboxylic acid cycle. The type of cell, or more likely cells, influenced by these compounds was not elucidated, nor was the site of action in the animal reported. Infected animals at the time of death exhibited the same microbial titers as those found in untreated animals,8 but did demonstrate a more rapid and pronounced bacteremia following intraperitoneal injection. ' Leucocytic function as assayed by the smear technique on blood granulocytes was within a normal range, and this resistance mechanism was not considered to be an influential factor in accelerating the infectious process. ' More recently, other investigators have reported that malonate interferes with the intracellular killing of gram-negative bacteria by mouse peritoneal macrophages.' In view of the important alterations in host resistance brought about by these agents, a study was conducted aimed at elucidating the mechanisms involved in the enhanced susceptibility to staphylococcal infection of mice treated with metabolic inhibitors.
Source: PubMed Central
Author: Z A COHN
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2297795709