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The Use of Hybrid Somatic Cells as an Approach to Mitochondrial Genetics in Animals

Year: 1974

Type: article

Abstract: We have studied the fate of parental mitochondrial DNA (mtDNA) in hybrid somatic cells derived by Sendai virus-induced fusion of human cells and mouse or rat cells. Many hybrid cell strains were obtained which contained sequences from both human and rodent mtDNA after 40 to 60 population doublings. Some strains were subcloned and cultured further for up to 150 doublings; a large fraction of these strains contained both parental mtDNA sequences at that time. The relation between human and rodent mtDNA sequences was tested in some of the hybrid cell strains. In a high fraction of strains tested the human and rodent mtDNA sequences were linked to each other by what are most likely covalent bonds. This linkage may be described as "recombination" of mtDNA sequences from two different animals.

Source: Genetics

DOI: 10.1093/genetics/78.1.459

Authors: Igor B Dawid, Ivan Horak, Hayden G Coon

Institutions: Carnegie Institution for Science, Department of Embryology, National Cancer Institute

Language: English

Topic: Mitochondrial Function and Pathology

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W2298986814

FWCI: 0.982

Cites:

Cited by: 18

Related to: 10