Work

Quantitative analysis of serotonin secreted by human embryonic stem cells‐derived serotonergic neurons via pH‐mediated online stacking‐CE‐ESI‐MRM

Year: 2016

Type: article

Abstract: A CE-ESI-MRM-based assay was developed for targeted analysis of serotonin released by human embryonic stem cells-derived serotonergic neurons in a chemically defined environment. A discontinuous electrolyte system was optimized for pH-mediated online stacking of serotonin. Combining with a liquid-liquid extraction procedure, LOD of serotonin in the Krebs'-Ringer's solution by CE-ESI-MS/MS on a 3D ion trap MS was0.15 ng/mL. The quantitative results confirmed the serotonergic identity of the in vitro developed neurons and the capacity of these neurons to release serotonin in response to stimulus.

Source: Electrophoresis

DOI: 10.1002/elps.201500496

Authors: Xuefei Zhong, Ling Hao, Jianfeng Lu, Hui Ye, Su‐Chun Zhang, Lingjun Li

Institutions: University of Wisconsin–Madison, China Pharmaceutical University, Tianjin University

Language: English

Topic: Microfluidic and Capillary Electrophoresis Applications

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Neurological Disorders and Stroke