Metagenomic Sequencing with Strain-Level Resolution Implicates Uropathogenic E. coli in Necrotizing Enterocolitis and Mortality in Preterm Infants
Year: 2016
Type: article
Study design: Observational Study
Abstract: Necrotizing enterocolitis (NEC) afflicts approximately 10% of extremely preterm infants with high fatality. Inappropriate bacterial colonization with Enterobacteriaceae is implicated, but no specific pathogen has been identified. We identify uropathogenic E. coli (UPEC) colonization as a significant risk factor for the development of NEC and subsequent mortality. We describe a large-scale deep shotgun metagenomic sequence analysis of the early intestinal microbiome of 144 preterm and 22 term infants. Using a pan-genomic approach to functionally subtype the E. coli, we identify genes associated with NEC and mortality that indicate colonization by UPEC. Metagenomic multilocus sequence typing analysis further defined NEC-associated strains as sequence types often associated with urinary tract infections, including ST69, ST73, ST95, ST127, ST131, and ST144. Although other factors associated with prematurity may also contribute, this report suggests a link between UPEC and NEC and indicates that further attention to these sequence types as potential causal agents is needed.
Source: Cell Reports
Language: English
Topic: Infant Nutrition and Health
Subfield: Nutrition and Dietetics
Field: Nursing
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $5,620
Funders: U.S. Department of Health and Human Services, Broad Institute, European Commission, Ministero dell’Istruzione, dell’Università e della Ricerca, Università degli Studi di Trento, Fondazione Cassa Di Risparmio Di Trento E Rovereto, National Institutes of Health, Seventh Framework Programme, Research Executive Agency, National Human Genome Research Institute, National Institute of Allergy and Infectious Diseases, National Center for Research Resources, National Center for Advancing Translational Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development, FP7 People: Marie-Curie Actions
Awards: G1029946750, G1044601524, Novel genetic and salivary glycan biomarkers for risk of NEC in ELBW infants., Defining the Human Microbiome, Cincinnati Center for Clinical and Translational Sciences and Training, G325765397, NICHD Cooperative Multicenter Neonatal Research Network, Center for Clinical and Translational Science and Training, G5729080129, The Role of Human Milk in Infant Nutrition and Health, G6007859745, G6579334312, G9000300183, NICHD Neonatal Research Network -- Cincinnati Clinical Center
OpenAlex ID: W2302391071