Year: 2010
Type: article
Abstract: Molecular recognition is an integral part of several biological processes and systems and occurs between cells, proteins and nucleic acids.The molecular recognition between leukocytes and endothelial cells direct the migration of leukocytes from the blood to sites of pathological inflammation.Molecular recognition between proteins and nucleic acids are utilized in various molecular biology techniques such as enzyme-linked immunosorbent assays (ELISA) and quantitative real time polymerase chain reactions (q-PCRs).Leukocyte -endothelial interactions play a key role in pathological inflammation and blocking such interactions would help in preventing the advancement of the disease.Several studies have shown that E-selectin, expressed on inflamed endothelium, and proteins and/or lipids decorated with sialyl Lewis x (sLe x ), present on the surface of leukocytes, play a major role in the initial leukocyte -endothelial interactions.It is reported that the monoclonal antibody (mAb) HECA-452 recognizes sLe x .This observation motivated us to determine if the mAb HECA-452 blocks sLe x mediated adhesion to endothelial expressed E-selectin.The results of this study showed that HECA-452 recognizes sLe x and does not block sLe x mediated adhesion to endothelial expressed E-selectin.HECA-452 recognition of sLe x does not depend on the fucose moiety to the extent required for E-selectin recognition.Combined, this study suggests Dr. David F. J. Tees for their timely support and technical input.
Author: China Malakondaiah Kummitha
Institution: Ohio University
Language: English
Subfield: Immunology and Allergy
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2303817780