Characterisation of a new, fully active fluorescent derivative of E. coli tRNA Phe.
Year: 1980
Type: article
Abstract: E. coli tRNAPhe has been labelled with fluorescein isothiocyanate taking advantage of the reactivity of this compound for primary aliphatic amino groups as exist in this tRNA as the modified base X(3-(3-amino-3-carboxypropyl)uracil). The extent of labelling was calculated as 1.6 nmole/A260 unit suggesting one dye molecule per tRNA. The FITC-tRNA showed full activity in aminoacylation and polypeptide synthesis. The absorption and fluorescence of the label respond markedly on addition of Mg++ to the tRNA. The label appears to be a sensitive probe of tRNAPhe tertiary structure.
Source: PubMed Central
Authors: J A Plumbridge, H G Bäumert, M Ehrenberg, R Rigler
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2304884033