Effects of Tricine, Glycine and Tris Buffers on Alkaline Phosphatase Activity
Year: 2026
Type: article
Abstract: The effect of Tris, Glycine and Tricine on alkaline phosphatase activity was tested at a pH of 8.6, 8.85 and 9.1, using pNPP as substrate. Alkaline phosphatase activity was measured by monitoring the increase in NP absorbance at 420nm using a spectrophotometer. Alkaline phosphatase in Tris buffer at pH 9.1 was found to have the highest activity. Michaelis- Menten enzyme kinetic analysis revealed that the presence of Tris produced the highest V max and K values for alkaline phosphatase at each pH tested, while the presence of Glycine m produced the lowest. Also, increasing the pH was found to increase both the V and K. max m
Source: Open Collections
Authors: HETHEY, JAMES, LAI, JACQUELINE, LOUTET, SLADE, MARTIN, MORGAN, TANG, VINCENT
Institution: University of British Columbia
Language: English
Subfield: Endocrinology, Diabetes and Metabolism
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2305714288