Work

Photonic crystal protein hydrogel sensor materials enabled by conformationally induced volume phase transition

Year: 2016

Type: article

Abstract: glucose/galactose binding protein (GGBP). The resulting 2-D PC-GGBP hydrogel undergoes a VPT in response to glucose. The volume change causes the 2-D PC array particle spacing to decrease, leading to a blue-shifted diffraction which enables our sensors to report on glucose concentrations. This 2-D PC-GGBP responsive hydrogel functions as a selective and sensitive sensor that easily monitors glucose concentrations from ∼0.2 μM to ∼10 mM. This work demonstrates a proof-of-concept for developing responsive, "smart" protein hydrogel materials with VPTs that utilize ligand binding induced protein conformational changes. This innovation may enable the development of other novel chemical sensors and high-throughput screening devices that can monitor protein-drug binding interactions.

Source: Chemical Science

DOI: 10.1039/c6sc00682e

Authors: Zhongyu Cai, Linda A. Luck, David Punihaole, Jeffry D. Madura, Sanford A. Asher

Institutions: University of Pittsburgh, SUNY Plattsburgh, Duquesne University

Language: English

Topic: Photonic Crystals and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: diamond