Year: 2013
Type: article
Abstract: Ghrelin is a gastrointestinal hormone that activates the ghrelin receptor (GHSR) in hypothalamic neurons to promote feeding and peripheral lipid storage.Ghrelin contains a unique acyl modification that is esterified to the peptide molecule.The recent discovery of the ghrelin acylating enzyme, ghrelin O-acyltransferase (GOAT), has provided a deeper understanding of the acylation process and actions of acyl ghrelin (AG) versus des-acyl ghrelin (dAG).Our group previously demonstrated that GOAT utilizes various dietary fatty acids (FAs) as a substrate for ghrelin acylation.In the current study, we use both in vitro and in vivo approaches to demonstrate that ghrelin action on GHSR activation, food intake, body weight and fat mass can be modified by altering the FA side-chain length of the ghrelin peptide molecule.Additionally, we find that AG and dAG act in the central nervous system to increase glucose-stimulated plasma insulin levels indicating that this function of ghrelin is independent of its acylation state.Finally, we combine genetic and pharmacological approaches to demonstrate that the regulation of glucose tolerance by GOAT action is dependent on calorie restriction and age.Collectively, these data systematically dissect the function of ghrelin acylation in regulating energy and glucose metabolism.I would like to thank my thesis mentors, Matthias Tschöp and Randy Seeley, for all of their guidance during my graduate studies.You both have exceptional speaking and writing skills that I truly admire.Matthias, I feel very privileged to work with one of the top researchers in the ghrelin field.Randy, I am glad that you stepped in as my mentor when Matthias moved the lab to Germany.I appreciate all of the advice you gave me when I was searching for post-doc positions.You helped me reflect on what I wanted to do with my career and I am so happy
Author: Kristy M. Heppner
Institution: University of Cincinnati
Language: English
Subfield: Endocrine and Autonomic Systems
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2308109441