Year: 2011
Type: dissertation
Abstract: Fibroblasts in culture have been used as models for the study of aging.Under standard culture conditions or after the action of known stressors as oxidants, cells exhibit several features of which cell cycle arrest is the loss-of-function hallmark.Resveratrol, shown to prevent age-related effects in different organisms, is currently under active study and is employed in cell ageing models.Early passage WI-38 fibroblasts grown in medium with or without resveratrol were submitted to subcytotoxic levels of H 2 O 2 .Cell proliferation, measured by MTT assays for day 2 upon to day 5, exhibited a dose dependent reduction, which was intensified by H 2 O 2 .Similarly, senescence associatedβ -galactosidase (SA-β-gal) positively stained cells increased.Resveratrol attenuated H 2 O 2 cytotoxic effect but enhanced sirtuin 1 expression, manganese superoxide dismutase, p21 and Cyclin D1.Exposure to H 2 O 2 resulted in SIRT1 expression decrease but the effect was reverted in cells pre-treated with resveratrol.In H 2 O 2 -induced SIPS, resveratrol blunted MnSOD, p21 and Cyclin D expression.The results show that resveratrol results in the expression of features resembling replicative senesncence, which are enhanced by the exposition to H 2 O 2 .
DOI: 10.34626/t32j-aq49
Author: Seabra, Fátima Leal
Language: English
Subfield: Geriatrics and Gerontology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: Banco Santander, Universidade do Porto
OpenAlex ID: W231185638