Year: 1996
Type: article
Abstract: CRYSTALLOGRAPHY OF BIOLOGICAL MACROMOLECULESOld yellow enzyme (OYE) (E.C. 1.6.99.1) was the first identit!edflavoprotein and has been used as a model protein in the studies of 11avoprotein and 11avin chemistry.However its physiological function still remains elusive.Recent discoveries that 01:c interacts with actin protein and deletion of OYE genes in S. cerevisiae leads to perturbed actin cytoskeleton indicate that OYE plays an important role in yeast.The structure of old yellow enzyme from brewer's yeast (OYE 1) was solved to 2.0A resolution, showing a TIM-barrel domain structure.We have grown crystals of OYE2.an OYE isoform from S. Cerevisiae.The crystals belong to space group P212121 (a=70A.b=96A, c= 151A) and diffract to 2.5A resolution.The structure detennination is in progress by molecular replacement and we plan to report a comparison between the structures of OYE1 and OYE2 isoforms.PS04.01.
Authors: K. Q. Yue, N. Croteau, A. Vrielink
Institutions: University of Milan, University of Pavia, University of Genoa
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2314614384