Work

Bromodeoxyuridine (BrdU) labeling and immunohistochemical detection in adult zebrafish brain

Year: 2013

Type: article

Abstract: Zebra sh is an ideal model organism to study adult neurogenesis.New neurons are born in various parts of zebra sh brain all through its lifespan.Analyzing neuron renewal in adult brain provides a valuable insight into neurodegenerative diseases.Bromodeoxyuridine \(BrdU) is a thymidine analog and it incorporates into the DNA only if the cells are at S-phase.BrdU labeling is a reliable method to locate the cells that are actively dividing.Here we describe the steps required for BrdU labeling in adult zebra sh brain starting from BrdU injection to the nal analysis in the microscope.We utilize a paraformaldehyde xation, vibratome slicing, uorescent labeling of incorporated BrdU and nally confocal imaging.For a thorough coverage on the topic zebra sh neurogenesis, readers are referred to \ [1] from which this protocol is adapted.A protocol on diverse applications of BrdU labeling can be found in protocol series by Jackson and Cook \[2] \[3] \[4].

Source: Protocol Exchange

DOI: 10.1038/protex.2013.087

Authors: Ayca Arslan Ergul, Ayca Arslan Ergul, Dilara Özge Halim, Michelle M Adams

Institution: Bilkent University

Language: English

Topic: Neurogenesis and neuroplasticity mechanisms

Subfield: Developmental Neuroscience

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Funder: Türkiye Bilimsel ve Teknolojik Araştırma Kurumu

Award: Zebra balığı modellerinde yaşlanma sürecinde nörogenezis karşılaştırması