Putative role for the GTPase, hGBP-1, in tumor cell proliferation and resistance to paclitaxel
Year: 2014
Type: article
Abstract: Cell proliferation and cell death are essential, yet opposing, cellular processes that maintain homeostasis.Disruption of these programmed cellular processes may lead to cancer progression, often accompanied by resistance to chemotherapy.During cancer progression, interferons production can lead to the induction of human Guanylate-Binding Protein-1 (hGBP-1).hGBP-1 retards the proliferation of endothelial cells but its role in carcinomas is not fully understood.The present study demonstrates that ectopic expression of hGBP-1 inhibits the proliferation of MCF-7 breast cancer and 2fTGH sarcoma cells, but its expression in DU145 prostate cancer cells does not influence their proliferation.In contrast, IFN-γ or IFN-2αA treatment does not influence the proliferation of MCF-7 and 2fTGH cells, but the proliferation of DU145 cells is inhibited by IFN-α2a treatment.This indicates that interferons do not universally inhibit the proliferation of carcinomas.Some breast and ovarian cancer cell lines show elevated expression of hGBP-1 when they are made resistant to paclitaxel.The ectopic expression of hGBP-1 in OVCAR8 cells protects the cells from paclitaxel-induced cytotoxicity.This protection by hGBP-1 is also provided in MCF-7 breast cancer and and encouraged me as well as given me constructive comments along the way.I have been extremely lucky to have a supervisor who cared so much about my work, and who responded to my questions and queries so promptly.I would also like to acknowledge and thank the University of Toledo for selecting me in the program and providing me with assistance for this study.A special thanks to the Biological Science Department Chair, Dr. Douglas Leaman, whose appreciation inspired me to complete my degree.My sincere thanks go to all of my past and present labmates for their support.I would especially like to thank Suzan Wadi
Author: Shilpi Chowdhury
Institution: University of Toledo
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2315603450