Work

Reprogramming efficiency of mouse embryonic fibroblasts using pMX-based retroviral expression vectors: testing factors that enhance efficiency.

Year: 2013

Type: article

Abstract: This protocol describes the testing of factors that can enhance the reprogramming of mouse embryonic broblasts \(MEFs) to iPC cells using pMX-based retroviral expression. ProcedureNote: The success of this experiment is dependent on proper growth of MEFs and high retroviral titers.It is important to take all notes in this protocol into account.Day 1 -evening: plate 293T cells in MEF medium at 2.2 x 10e6 cells per 10 cm dish. 1 dish per transfection.Separate viral supernatants from

Source: Protocol Exchange

DOI: 10.1038/protex.2013.035

Authors: W.W.M. Pim Pijnappel, W.W.M. Pim Pijnappel, Daniel Esch, Hans R. Schöler, H.T.Marc Timmers

Language: English

Topic: Pluripotent Stem Cells Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

OpenAlex ID: W2317933159

FWCI:

Cites: 1

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Related to: 10