Mp60-20 Estrogen Replacement Reduces Oxidative Stress and Restores Bladder and Pelvic Floor Contractile Function in an Animal Model of Menopause and Tissue Ischemia
Year: 2016
Type: article
Abstract: You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology I1 Apr 2016MP60-20 ESTROGEN REPLACEMENT REDUCES OXIDATIVE STRESS AND RESTORES BLADDER AND PELVIC FLOOR CONTRACTILE FUNCTION IN AN ANIMAL MODEL OF MENOPAUSE AND TISSUE ISCHEMIA Amy D. Dobberfuhl, Catherine Schuler, Robert E. Leggett, Elise J.B. De, and Robert M. Levin Amy D. DobberfuhlAmy D. Dobberfuhl More articles by this author , Catherine SchulerCatherine Schuler More articles by this author , Robert E. LeggettRobert E. Leggett More articles by this author , Elise J.B. DeElise J.B. De More articles by this author , and Robert M. LevinRobert M. Levin More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.02.873AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Surgical menopause following ovariectomy (OVX) in the rabbit has been shown to cause genitourinary hypoxia, decreased blood flow to the bladder and uterus, thinning of bladder mucosa, urothelial hypoxia and poor bladder compliance. In addition to estrogen depletion, clinical evidence in humans suggests that cyclic bladder ischemia, detrusor oxidative stress and pelvic floor ischemic dysfunction may contribute to the lower urinary tract symptoms which emerge at menopause. To further elucidate this relationship, we investigated the effect of estrogen depletion and replacement on the contractile response of bladder detrusor (BDM) and pelvic floor (PFM) musculature in the setting of in-vitro ischemia-reperfusion. METHODS Twelve female adult rabbits were divided into three groups: control, OVX and OVX with estradiol (E) replacement. At 4 weeks animals were euthanized. BDM, pubococcygeus and coccygeus were isolated into 150 mg strips. Tissues were equilibrated with oxygenated Tyrodes containing glucose, stimulated with field stimulation, and then stimulated under ischemic conditions for 1 hour in nitrogenated Tyrodes without glucose. Tissues were then incubated in oxygenated Tyrodes for 2 hours and stimulated. RESULTS Bladder weight was significantly decreased after OVX and restored above control values after E replacement. Estrogen replacement in OVX rabbits improved the ischemic contractile response of BDM and PFM above baseline levels during the entire 60 minute period of ischemia (Figure 1). Improvement in the contractile response of both BDM and the PFM was significantly greater in OVX animals after E replacement during the period of post-ischemia reperfusion (Figure 2). Markers of oxidative stress were concordant with the improvement of contractile function following E replacement. CONCLUSIONS Replacement of E after OVX reduced the oxidative stress on tissue and was protective to the effects of ischemia on pelvic floor and bladder contractile function. © 2016FiguresReferencesRelatedDetails Volume 195Issue 4SApril 2016Page: e802 Advertisement Copyright & Permissions© 2016MetricsAuthor Information Amy D. Dobberfuhl More articles by this author Catherine Schuler More articles by this author Robert E. Leggett More articles by this author Elise J.B. De More articles by this author Robert M. Levin More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
Source: The Journal of Urology
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2317944725