Work

Intramolecular Hydrogen Transfer Reactions of Thiyl Radicals from Glutathione: Formation of Carbon-Centered Radical at Glu, Cys, and Gly

Year: 2012

Type: article

Abstract: Glutathione thiyl radicals (GS(•)) were generated in H(2)O and D(2)O by either exposure of GSH to AAPH, photoirradiation of GSH in the presence of acetone, or photoirradiation of GSSG. Detailed interpretation of the fragmentation pathways of deuterated GSH and GSH derivatives during mass spectrometry analysis allowed us to demonstrate that reversible intramolecular H-atom transfer reactions between GS(•) and C-H bonds at Cys[(α)C], Cys[(β)C], and Gly[(α)C] are possible.

Source: Chemical Research in Toxicology

DOI: 10.1021/tx3000494

Authors: Olivier Mozziconacci, Todd D. Williams, Christian Schöneich

Institution: University of Kansas

Language: English

Topic: Redox biology and oxidative stress

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute on Aging

Awards: G2878525540, Reactive Oxygen Species and Aging