Partially Purified Pigment Extract from Streptomyces A 16-1 Induces Apoptosis of Human Carcinoma of Nasopharynx Cell (KB cells) via the Mitochondrial and Caspase-3 Pathway
Year: 2016
Type: article
Abstract: The genus Streptomycetes are very important bacteria which produce secondary metabolites used in clinical cancer therapy and other pharmacological activities. Streptomyces A 16-1 was isolated from coastal soil in Chonburi, Thailand. The objective of this study is to examine the apoptotic effects and mechanisms of the partially purified pigment extract from Streptomyces A 16-1 against human carcinoma of nasopharynx cell (KB cells). The methanol and ethyl acetate extract from the fermentation of ISP2 broth was fractionated by using column chromatography with stationery-phase silica-gel and mobile phase chloroform: methanol mixtures with increasing polarity. Among 9 fractions (Fr), the fractions which contained red pigment were selected and the calculated IC50 values for Fr 5, Fr 6 and Fr 7 were 0.04 ± 0.005, 0.20 ± 0.02, and 0.55 ± 0.05 μg/ml, respectively. The molecular mechanisms of cell death were associated with DNA fragmentation, accumulation of cells in sub-G1 phase, mitochondrial membrane depolarization, and activation of caspase-3, which was significantly diminished by the caspase-3 inhibitor. These first findings demonstrate that the pro-apoptotic effects of these fractions were mediated through the mitochondrial and caspase-3 pathway.
Authors: Chantarawan Saengkhae, Rattanaporn Srivibool, Rawiwan Watanadilok, Keiichi Enomoto
Institutions: Burapha University, Kochi University of Technology
Language: English
Subfield: Biotechnology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2319723643