Work

Catch and Release DNA Decoys: Capture and Photochemical Dissociation of NF-κB Transcription Factors

Year: 2016

Type: article

Abstract: Catch and release DNA decoys (CRDDs) are a new class of non-natural DNA probes that capture and dissociate from DNA-binding proteins using a light trigger. Photolytic cleavage of non-natural nucleobases in the CRDD yields abasic sites and truncation products that lower the affinity of the CRDD for its protein target. Herein, we demonstrate the ability of the first-generation CRDD to bind and release NF-κB proteins. This platform technology should be applicable to other DNA-binding proteins by modification of the target sequence.

Source: ACS Chemical Biology

DOI: 10.1021/acschembio.6b00130

Authors: Nicholas B. Struntz, Daniel A. Harki

Institution: University of Minnesota

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green