Pd20-12 Development of a Clinically Relevant Symptom Index to Assess Patients With Chronic Orchialgia
Year: 2016
Type: article
Abstract: INTRODUCTION AND OBJECTIVES: Indoleamine 2, 3-dioxygenase (IDO) catalyzes the first and rate-limiting step of tryptophan catabolism and has been implicated in immune tolerance.IDO is known to be induced in various tissue during systematic bacterial infection and play a key role in immune response.In our previous research, we elucidated that epididymal IDO expression in the mouse is restricted to the caput region from segments 2 to 5 with peak of expression in segments 3 to 4. However, the role of IDO in epididymitis still remains unclear.We investigated all sorts of cytokines and analyzed specific function of IDO in epididymitis model using IDO knock out mouse in this study.METHODS: Twelve weeks old C57BL/6J (wild type: WT and IDO knock-out: IDO KO) male mice were used in this study.Mice were injected with lipopolysaccharide (LPS) 4mg/g (weight) into epididymis on the side of spermatic code.At 7 days after LPS injection, epididymis were removed and examined histologically and biochemically by using cytokine assay (ELISA).Histological finding and expression of cytokines, their experiments were duplicated at least.RESULTS: Regarding HE stain, destruction of epididymal ductal structure and invasion of lymphocyte-predominant inflammatory cells were found in epididymitis model of IDO KO mice compared with WT mice.Comprehensive cytokine assay (ELISA) showed that both chemokines (CCL2, CCL3, CCL4, CCL5, CXCL10) and inflammatory promoting cytokines (IL-1ß, IL-2, IL-6, IL-12p70, IL-17, IL-23) were down-regulated in epididymitis model of IDO KO mice compared with WT mice.On the other hand, inflammatory inhibiting cytokines (IL-4, IL-10) were up-regulated.Next, we determined the quantity of IL-6 (ELISA), which is representative marker of inflammatory promoting cytokine.Significant decreased IL-6 expression was statistically observed in epididymitis model of IDO KO mice compared with WT mice (P<0.05).CONCLUSIONS: IDO is involved in epididymal inflammation by LPS.IDO might be a novel target for the therapy of the epididymitis in addition to antibodiotics.
Source: The Journal of Urology
Language: English
Subfield: General Health Professions
Field: Health Professions
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2322419273