Work

VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy

Year: 2015

Type: article

Abstract: VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus, induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.

Source: Journal of Microbiology and Biotechnology

DOI: 10.4014/jmb.1501.01007

Authors: Mi-Ae Lee, Jeong-A Kim, Mee-Young Shin, Jeong K. Lee, Soon-Jung Park, Kyu-Ho Lee

Institutions: Sogang University, Yonsei University, Yonsei University Health System

Language: English

Topic: Cell death mechanisms and regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Ministry of Food and Drug Safety