Year: 1988
Type: article
Abstract: Phenylalanine ammonia-lyase (PAL) was purified from bamboo shoots (Bambusa oldhami Munro) through steps of DEAE-Sephacel column chromatography, Sepharose CL-6B gel filtration, and a second DEAE-Sephacelcolumn chromatography.Polyacrylamide gel electrophoresis showed that the purified enzyme (Mr 360,000) still contained two minor bands with much slower mobilities (Mr ^660,000) than the major one.The minor band could be the aggregated forms of native PAL, based on the fact that only a single band (M, 86,000) was observed upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis or isoelectric focusing, and all bands shared common antigenic determinants as revealed by immunoblotting done with an antiserum raised against the major band.There was a sharp rise of PALactivity of bamboo shoots after harvest, and this was only partly due to the de novo synthesis of this enzyme as measured by Laurell's rocket immunoelectrophoresis.
Authors: Ru-Yin Chen, Tsung-Chain Chang, Ming-Sai Liu
Institution: Food Industry Research and Development Institute
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2323677152