Work

A protocol for imaging spindle orientation and inheritance of fate determinants in real time.

Year: 2014

Type: article

Abstract: This protocol describes a strategy to image spindle orientation during cell division in hematopoietic stem and progenitor cells in real time.This live imaging method was a modi cation of a method previously used to visualize the spindle using epithelial cell lines \(reference 1).This protocol also describes a strategy to track the orientation of the mitotic spindle coordinately with inheritance of the cell fate determinant Numb in real time.

Source: Protocol Exchange

DOI: 10.1038/protex.2014.014

Authors: Bryan Zimdahl, Bryan Zimdahl, Jeevisha Bajaj, Tannishtha Reya

Institution: University of California San Diego

Language: English

Topic: Microtubule and mitosis dynamics

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

OpenAlex ID: W2323936138

FWCI:

Cites: 1

Cited by:

Related to: 10