Year: 2014
Type: article
Abstract: The stop-and-go-extraction tips \(StageTips) have been widely used in shotgun proteomics to clean/desalt peptide samples prior to LC-MS/MS analysis.Here, an extremely simple and high throughput StageTip protocol is described.In this protocol, an adaptor is introduced to the StageTip, and makes it readily available for bench-top centrifugation.Each spin step \(with 200 μL buffer loaded) takes around 2 min at 4,000 rpm.Compared with previous syringe-based manual pressure device, the spinnable StageTip is completely labor-free, automatable, and can be easily scaled up.Considered with the recently developed 96FASP method, the spinnable StageTips provide another component to the high throughput work ow for clinical proteomics and biomarker discoveries.
Source: Protocol Exchange
Institution: J. Craig Venter Institute
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W2324005333