Year: 2011
Type: article
Abstract: Poster Sessions C590 amplified by PCR introducing in all of them the same double restriction sites present in a library of expression vectors, which will add different tags (MBP, GB1, His-tag at the N-or C-terminus) to each construct.All constructs are subsequently tested for bacterial overexpression using different strains and at different culture conditions.The last step consists of a solubility screening for each construct tested at a specific strain and expression condition, by using different buffers designed according to a semi-rational approach.The results from different analysis will be presented.
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2324043152