Viscoelastic properties of collagen hydrogels
Year: 2016
Type: article
Abstract: Event Abstract Back to Event Viscoelastic properties of collagen hydrogels Bernard Drouin1, 2, Lucie Lévesque1, Audrey Lainé1, Erica Rosella1, Caroline Loy1 and Diego Mantovani1 1 Laval University, Lab for Biomaterials and Bioengineering (CRC-1) Dept. Min-Met-Materials and Chu de Quebec Res Center, Canada 2 Cegep Garneau, Physics Dept., Canada Introduction: Because of their exceptional biological properties, reconstituted collagen gels are widely used as scaffolds even though their low elastic modulus (~ 500 Pa) limits their applications[1],[2]. The mechanical properties of collagen are governed by viscoelasticity. Unfortunately, few reports deal the mechanical properties of hydrogels that need to be addressed to understand the behavior of suchhydrogels when submitted to either creep-like or relaxation-like mechanical testing. Materials and Methods: Type I collagen was extracted from rat-tail tendons and solubilized in acetic acid solution (0.02N) at 4 g/L as previously reported[3]. Unconfined compression tests were made on disk shaped samples with a 25 mm compression plate attached to a 150 gf load cell mounted on a Biomomentum Mach 1 (Laval, Canada) testing apparatus. Compression tests were performed at room temperature in Phosphate-Buffered Saline (PBS) solution. Viscoelastic models[4] based on two time constants Maxwell and standard linear solid were used. In addition, to reconcile experimental results and theory nonlinear stress-strain relationship based on Neo-Hookean and Mooney-Rivlin models were used. Results: Figure 1 shows a typical multiple relaxation test obtained with three samples: a water drop, a native collagen hydrogel and a collagen gel that has been colonized with smooth muscle cells for one week. The load of the water drop is increasingly oriented toward the tension side (negative) of the load curve due to increased surface tension force. The load curve of the native collagen gel is almost neutral and slightly toward the negative part. This indicates that the collagen native hydrogel exhibits no real residual stress when compressed for many steps. The viscoelastic model of such a material necessitates the use of Maxwell bodies. The other curve shows a residual stress that increases with the depth of the compression. In this case, the standard linear solid viscoelastic model is used. In figure 2, only the compression phase is illustrated. The simulation curves to reproduce the experimental result are indicated. Using a single Maxwell body, one can reproduce the experimental load if two conditions are met. The first condition requires a long time constant (~ 150s). As illustrated, a fast time constant is not adequate to reproduce the results. The second condition requires that a nonlinear relationship between Load and strain be used. In this case, a neo-hookean approach is used. Only in this combination of parameters, can one hope to reproduce experimental tests with simulation using standard viscoelastic models. Conclusion: This paper will present the results of many tests performed on collagen gels and present the conclusions regarding the viscoelastic models that should be retained when dealing with collagen gels mechanical properties. NSERC CREATE; NSERC-Canada; FRQ-S; FRQ-NTReferences:[1] Sander et al., Collagen: Structure and Mechanics, P. Fratzl (ed.), ch 17, Springer, 2008[2] Seifu et al., Nature Reviews Cardiology, 2013, 10, 7, 410[3] Rajan, N; Habermehl, J; Coté, M.-F; Doillon, C.J; Mantovani, D., Nature Protocols, 2006,1,2753-8.[4] Fung, Biomechanics: Mechanical properties of living tissues, 2nd ed. 1993 Keywords: Hydrogel, modeling, Scaffold, mechanical property Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Mechanical properties of biomaterials Citation: Drouin B, Lévesque L, Lainé A, Rosella E, Loy C and Mantovani D (2016). Viscoelastic properties of collagen hydrogels. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00535 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Bernard Drouin Lucie Lévesque Audrey Lainé Erica Rosella Caroline Loy Diego Mantovani Google Bernard Drouin Lucie Lévesque Audrey Lainé Erica Rosella Caroline Loy Diego Mantovani Google Scholar Bernard Drouin Lucie Lévesque Audrey Lainé Erica Rosella Caroline Loy Diego Mantovani PubMed Bernard Drouin Lucie Lévesque Audrey Lainé Erica Rosella Caroline Loy Diego Mantovani Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Authors: Drouin Bernard, L�vesque Lucie, Lain� Audrey, Rosella Erica, Loy Caroline, Mantovani Diego
Language: English
Subfield: Biomaterials
Field: Materials Science
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W2324271117