Work

Fatty Acid Hydrolysis of Acyl Marinobactin Siderophores by Marinobacter Acylases

Year: 2015

Type: article

Abstract: The marine bacteria Marinobacter sp. DS40M6 and Marinobacter nanhaiticus D15-8W produce a suite of acyl peptidic marinobactin siderophores to acquire iron under iron-limiting conditions. During late-log phase growth, the marinobactins are hydrolyzed to form the marinobactin headgroup with release of the corresponding fatty acid tail. The bntA gene, a homologue of the Pseudomonas aeruginosa pyoverdine acylase gene, pvdQ, was identified from Marinobacter sp. DS40M6. A bntA knockout mutant of Marinobacter sp. DS40M6 produced the suite of acyl marinobactins A-E, without the usual formation of the marinobactin headgroup. Another marinobactin-producing species, M. nanhaiticus D15-8W, is predicted to have two pvdQ homologues, mhtA and mhtB. MhtA and MhtB have 67% identical amino acid sequences. MhtA catalyzes hydrolysis of the apo-marinobactin siderophores as well as the quorum sensing signaling molecule, dodecanoyl-homoserine lactone. In contrast to hydrolysis of the suite of apo-marinobactins by MhtA, hydrolysis of the iron(III)-bound marinobactins was not observed.

Source: Biochemistry

DOI: 10.1021/bi5013673

Authors: Michelle P. Kem, Hiroaki Naka, Akira Iinishi, Margo G. Haygood, Alison Butler

Institutions: University of California, Santa Barbara, Oregon Health & Science University

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: National Science Foundation, Oregon Health and Science University, Division of Chemistry

Awards: Microbial Iron Acquisition: Investigations of Amphiphilic Siderophores, G2545904804, G2640277711, Amphiphilic Siderophores: Biosynthesis, Iron Uptake and Effect on Microbial Growth