Work

Mp07-15 Utility of Pten/erg Status in a Contemporary Cohort of Men With Favorable-risk Prostate Cancers

Year: 2016

Type: article

Abstract: INTRODUCTION AND OBJECTIVES: In this study, we set up a real-time quantitative polymerase chain reaction (RT-qPCR) method to detect epithelial-mesenchymal transition (EMT) and stem cell gene expression status in peripheral blood to validate whether they could complement CTC enumeration in predicting the prognosis of metastatic castration-resistant prostate cancer (mCRPC).METHODS: From January 2013 to June 2014, we collected peripheral blood from 70 mCRPC patients and enumerated CTC in these blood samples using CellSearch system.At the same time, stem cell-related genes (ABCG2, PROM1 and PSCA) and EMT-related genes (TWIST1 and vimentin) were detected in peripheral blood samples using RT-qPCR assays.Patient overall survival (OS) and treatment response status were recorded in the follow-up.RESULTS: Unfavorable CTC enumerations were predictive of shorter OS (p ¼ 0.01).Also, positive stem cell gene expression indicated poor prognosis in mCRPC patients (p ¼ 0.01).A multivariate analysis indicated that serum albumin, ECOG performance status, CTC enumeration and stem cell expression status were independent prognostic factors for OS.For the 40 patients categorized into the favorable CTC enumeration group, positive stem cell gene expression also suggested poor prognosis (p ¼ 0.00).A combined prognostic model consisting of stem cell gene expression and CTC enumeration increased the concordance probability estimated value from 0.716 to 0.889 in comparison with CTC enumeration alone.For patients who received docetaxel plus prednisone as firstline chemotherapy, positive stem cell gene expression suggested a poor PSA-PFS (p ¼ 0.01) and a low PSA response rate (p ¼ 0.008).CONCLUSIONS: Detection of peripheral blood stem cell gene expression could complement CTC enumeration in predicting OS and docetaxel-based treatment effects in mCRPC patients.

Source: The Journal of Urology

DOI: 10.1016/j.juro.2016.02.2218

Authors: Jeffrey Tosoian, Carlos Morais, Liana Guedes, Abdelrazak Meliti, Stephanie Glavaris, Ridwan Alam, Meera Chappidi, Patrick Mullane, Edward Schaeffer, H. Ballentine Carter, Angelo DeMarzo, Ashley Ross, Tamara Lotan

Language: English

Topic: Prostate Cancer Treatment and Research

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2326494458

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