Work

Selective Detection and Quantification of Modified DNA with Solid-State Nanopores

Year: 2014

Type: article

Abstract: We demonstrate a solid-state nanopore assay for the unambiguous discrimination and quantification of modified DNA. Individual streptavidin proteins are employed as high-affinity tags for DNA containing a single biotin moiety. We establish that the rate of translocation events corresponds directly to relative concentration of protein-DNA complexes and use the selectivity of our approach to quantify modified oligonucleotides from among a background of unmodified DNA in solution.

Source: Nano Letters

DOI: 10.1021/nl501340d

Authors: Autumn T. Carlsen, Osama K. Zahid, Jan A. Ruzicka, Ethan W. Taylor, Adam R. Hall

Institutions: Virginia Tech - Wake Forest University School of Biomedical Engineering & Sciences, Wake Forest University, Virginia Tech, University of North Carolina at Greensboro

Language: English

Topic: Nanopore and Nanochannel Transport Studies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: North Carolina Biotechnology Center, Wake Forest University, University of Oxford

OpenAlex ID: W2327676286