Dextran functionalization enhances nanoparticle-mediated siRNA delivery and silencing
Year: 2016
Type: article
Abstract: Understanding the endocytosis and intracellular trafficking of short interfering RNA (siRNA) delivery vehicle complexes remains a critical bottleneck in designing siRNA delivery vehicles for highly active RNA interference (RNAi)-based therapeutics. In this study, we show that dextran functionalization of silica nanoparticles enhanced uptake and intracellular delivery of siRNAs in cultured cells. Using pharmacological inhibitors for endocytotic pathways, we determined that our complexes are endocytosed via a previously unreported mechanism for siRNA delivery in which dextran initiates scavenger receptor-mediated endocytosis through a clathrin/caveolin-independent process. Our findings suggest that siRNA delivery efficiency could be enhanced by incorporating dextran into existing delivery platforms to activate scavenger receptor activity across a variety of target cell types.
Source: TECHNOLOGY
Institution: Michigan State University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G172311494, Deep Sequencing to Screen Functional Antibody Epitopes, Engineering an in vitro model of adipose tissue formation and metabolism, Development of a parallel, array-based transcription factor expression assay, Phenotype-Targeted Inference of Flux-Enzyme Correlations in Adipocyte Metabolism, G3510797780, Develop a Dynamic Model that Incoporates Text-mining to Reconstruct Networks, G6490296102, G6543713385, G6908140774, G7615941542, G79308190, G7934200494, Maximizing siRNA Function through Mechanism-based Sequence and Vehicle Design
OpenAlex ID: W2327829505