Work

A method for culturing Gram-negative skin microbiota

Year: 2016

Type: article

Abstract: BACKGROUND: Commensal Gram-negative (CGN) microbiota have been identified on human skin by DNA sequencing; however, methods to reliably culture viable Gram-negative skin organisms have not been previously described. RESULTS: Through the use of selective antibiotics and minimal media we developed methods to culture CGN from skin swabs. We identified several previously uncharacterized CGN at the species level by optimizing growth conditions and limiting the inhibitory effects of nutrient shock, temperature, and bacterial competition, factors that may have previously limited CGN isolation from skin cultures. CONCLUSIONS: Our protocol will permit future functional studies on the influences of CGN on skin homeostasis and disease.

Source: BMC Microbiology

DOI: 10.1186/s12866-016-0684-9

Authors: Ian A. Myles, Jensen D. Reckhow, Kelli W. Williams, Inka Sastalla, Karen M. Frank, Sandip K. Datta

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases, National Institutes of Health Clinical Center

Language: English

Topic: Dermatology and Skin Diseases

Subfield: Dermatology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,990