Mycobacterium tuberculosis antigen-specific immune responses among tuberculosis lymphadenitis patients
Year: 2013
Type: article
Abstract: Event Abstract Back to Event Mycobacterium tuberculosis antigen-specific immune responses among tuberculosis lymphadenitis patients Kidist Bobosha1*, Liya Wassie1, Meseret Habtamu1, Martha Zewdie1, Jemal Hussein1, Lawrence Yamuah1 and Abraham Aseffa1 1 Armauer Hansen Research Institute, Ethiopia The reporting rate of extra-pulmonary TB (EPTB) has increased in most high TB burden countries in recent years. Tuberculosis lymphadenitis (TBLN) is one of the most common forms of EPTB and is considered as a local manifestation of a systemic infection. Evaluating the immune responses of TBLN patients to M. tuberculosis (MTB) specific antigens will provide important inputs to efforts being made towards developing efficient TB diagnostic tools and vaccines. In this study, peripheral blood mononuclear cells (PBMCs), from non-pulmonary TB but clinically, cytologically and/or culture and PCR confirmed TBLN patients (n=48) and healthy controls (n=23) were isolated and frequencies of antigen-specific (ESAT-6, CFP-10, RV2031c (ACR), Ag85B, TB10.4 and PPD) IFN-gamma producing T-cells (spot forming units/1 million PBMCs) were measured using ELISpot assay. Considering PHA as positive and media alone as negative control, the median frequencies of CFP-10- and TB10.4-specific IFN-gamma producing T-cells were significantly higher in TBLN patients compared to healthy controls (p < 0.001). Both groups showed poor responses to Ag85B. Strong responses to some of the selected TB antigens could demonstrate the role of the antigens in the identification of TBLN cases; however, more detailed analyses are warranted to satisfy the need for specific diagnosis of extra-pulmonary TB cases. Acknowledgements Dr. Mark Doherty from SSI for providing the antigens Financial support; AHRI core budget Keywords: TB lymphadenitis, Mycobacterium tuberculosis, Antigens, Elispot, IFN- γ Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Bobosha K, Wassie L, Habtamu M, Zewdie M, Hussein J, Yamuah L and Aseffa A (2013). Mycobacterium tuberculosis antigen-specific immune responses among tuberculosis lymphadenitis patients. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00649 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Mrs. Kidist Bobosha, Armauer Hansen Research Institute, Addis Ababa, Ethiopia, [email protected] Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kidist Bobosha Liya Wassie Meseret Habtamu Martha Zewdie Jemal Hussein Lawrence Yamuah Abraham Aseffa Google Kidist Bobosha Liya Wassie Meseret Habtamu Martha Zewdie Jemal Hussein Lawrence Yamuah Abraham Aseffa Google Scholar Kidist Bobosha Liya Wassie Meseret Habtamu Martha Zewdie Jemal Hussein Lawrence Yamuah Abraham Aseffa PubMed Kidist Bobosha Liya Wassie Meseret Habtamu Martha Zewdie Jemal Hussein Lawrence Yamuah Abraham Aseffa Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Source: Frontiers in Immunology
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W2329247334