Work

Community nominated projects at the Joint Center for Structural Genomics

Year: 2011

Type: article

Abstract: Poster Sessions C590 amplified by PCR introducing in all of them the same double restriction sites present in a library of expression vectors, which will add different tags (MBP, GB1, His-tag at the N-or C-terminus) to each construct.All constructs are subsequently tested for bacterial overexpression using different strains and at different culture conditions.The last step consists of a solubility screening for each construct tested at a specific strain and expression condition, by using different buffers designed according to a semi-rational approach.The results from different analysis will be presented.

Source: Acta Crystallographica Section A Foundations of Crystallography

DOI: 10.1107/s0108767311085084

Authors: Q. Xu, A. Deacon, I. A. Wilson

Institutions: SLAC National Accelerator Laboratory, Joint Center for Structural Genomics, Stanford Synchrotron Radiation Lightsource, Scripps Research Institute

Language: English

Topic: Enzyme Structure and Function

Subfield: Materials Chemistry

Field: Materials Science

Domain: Physical Sciences

Open Access status: bronze

Funders: U.S. Department of Energy, National Institutes of Health, National Institute of General Medical Sciences, National Center for Research Resources