Work

Promises and Pitfalls of Intracellular Delivery of Proteins

Year: 2014

Type: review

Abstract: The direct delivery of functional proteins into the cell cytosol is a key issue for protein therapy, with many current strategies resulting in endosomal entrapment. Protein delivery to the cytosol is challenging due to the high molecular weight and the polarity of therapeutic proteins. Here we review strategies for the delivery of proteins into cells, including cell-penetrating peptides, virus-like particles, supercharged proteins, nanocarriers, polymers, and nanoparticle-stabilized nanocapsules. The advantages and disadvantages of these approaches including cytosolar delivery are compared and contrasted, with promising pathways forward identified.

Source: Bioconjugate Chemistry

DOI: 10.1021/bc500320j

Authors: Ailing Fu, Rui Tang, Joseph Hardie, Michelle E. Farkas, Vincent M. Rotello

Institutions: Southwest University, University of Massachusetts Amherst

Language: English

Topic: RNA Interference and Gene Delivery

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500