Year: 2016
Type: article
Abstract: OBJECTIVES: One of pathogenesis of diabetic cystopathy is the disturbance of bladder microcirculation.We examined the effect of eicosapentaenoic acid (EPA) on bladder microcirculation and bladder overactivity using a diabetes rat model.METHODS: Twelve week-old female SD rats were divided into three groups; control, diabetes and diabetes + EPA groups.The latter two groups were injected with streptozotocin (65 mg/kg) intraperitoneally to induce diabetes.Blood glucose levels were measured at 3 days, and only rats with blood glucose >350 mg/dl were used in the study.EPA (mg/kg/day) or sunflower oil (control and diabetes groups) were orally given.At 2 or 8 weeks, rats were evaluated for bladder microcirculation, ELISA for oxidative stress markers, cystometrogram, and organ bath study.RESULTS: The blood flow on the bladder surface was disturbed in the diabetes rats, and improved in the diabetes + EPA group.Oxidative stress markers in the bladder (malondialdehyde) increased in the diabetes rats, which was ameliorated in the silodosin group.Cystometrogram demonstrated increased non-voiding contraction in the diabetes group, which was ameliorated by EPA treatment (8.7 Æ 1.7 in the control, 27.0 Æ 4.1 in the diabetes group, and 13.4 Æ 2.1 in the diabetes + EPA group).Organ bath study showed increased bladder contractility in diabetes rats compared to control, and EPA suppressed diabetes-induced hypercontractility.CONCLUSIONS: EPA treatment suppressed the disturbed microcirculation and overactivity of the bladder seen in the diabetes rats.
Source: The Journal of Urology
Authors: Michael Lam, Travis Mann-Gow, Peter Zvara
Language: English
Subfield: Urology
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
OpenAlex ID: W2331013646