Work

Disease Detection by Ultrasensitive Quantification of Microdosed Synthetic Urinary Biomarkers

Year: 2014

Type: article

Abstract: The delivery of exogenous agents can enable noninvasive disease monitoring, but existing low-dose approaches require complex infrastructure. In this paper, we describe a microdose-scale injectable formulation of nanoparticles that interrogate the activity of thrombin, a key regulator of clotting, and produce urinary reporters of disease state. We establish a customized single molecule detection assay that enables urinary discrimination of thromboembolic disease in mice using doses of the nanoparticulate diagnostic agents that fall under regulatory guidelines for "microdosing."

Source: Journal of the American Chemical Society

DOI: 10.1021/ja505676h

Authors: Andrew D. Warren, Shonda T. Gaylord, Kevin C. Ngan, Milena Dumont Milutinovic, Gabriel A. Kwong, Sangeeta N. Bhatia, David R. Walt

Institutions: Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Tufts University

Language: English

Topic: Advanced biosensing and bioanalysis techniques

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500

Funders: National Science Foundation, Howard Hughes Medical Institute, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, National Cancer Institute

Awards: G2360436881, Diagnostic peptide-nanoparticle probes for profiling tumor protease activity, G4774430914, G5108852245, G8863986071, Cancer Center Support (Core) Grant, G941982536

OpenAlex ID: W2331828106

FWCI: 2.436

Cites: 30

Cited by: 57

Related to: 10