Work

Population structure and molecular epidemiology of human clinical multi-drug resistant (MDR) Escherichia coli strains from Pune, India

Year: 2016

Type: article

Abstract: Background: Extraintestinal pathogenic E. coli (ExPEC) cause different infectious diseases in humans and animals, accounting 80% of urinary tract infections (UTIs), worldwide. Increase in the prevalence of multidrug-resistant (MDR) E. coli and global dissemination of these clonal organisms is a significant risk to the public health. The present study investigates the prevalence, population structure, phylogenetic affinities and molecular basis of ESBL and antimicrobial resistance and also highlights the spread of multi-drug resistant E. coli causing infections with varied clinical spectrum Methods & Materials: A total of 187 E. coli isolates from patients with different clinical complications were obtained from D.Y. Patil Medical College, Pune, for this study. All the isolates were subjected to phylogenetic grouping and specific sequence type (ST) identification. The ST131 strains were further evaluated for their virulence gene profiles using PCR. Phenotypic and genotypic detections of antimicrobial resistance were carried out by disc diffusion and PCR based methods, respectively. The clonality of ST131 isolates with respect to strains of other sequence types was evaluated by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Results: Phylogrouping of 187 E. coli isolates revealed that 78 (41.7%) isolates belonged to group A, 16 (8.6%) to group B1, 56 (29.9%) to group B2 and 37 (19.8%) to group D. Forty strains were identified to belong to ST131. Virulence profiling of ST131 strains demonstrated significant prevalence of fimH (90%), papC (82.5%), hlyA (77.5%) traT (47.5%), sat (87.5%) and iucD (55%). The antibiotic resistance profiles of ST131 isolates showed high resistance to ciproflaxin (92.5%), cotrimoxazole (67.5%) and tetracycline (62.5%). A high proportion (60%) of these ST131 strains was multidrug resistant and 95% were ESBL-positive. Of all the 40 ST131 strains, 42.5% and 95% were positive for sulphonamide and streptomycin resistance encoding genes, respectively, and only 2 strains were found to be positive for ndm-1. Conclusion: Our study revealed that ST131 is the only predominant endemic clone present in the clinical E. coli isolates from Pune. Molecular characterization of these isolates suggests that ST131 is a robust lineage of pathogen that could significantly limit medical interventions against E. coli induced enteric and extraintestinal infections in India.

Source: International Journal of Infectious Diseases

DOI: 10.1016/j.ijid.2016.02.741

Authors: N. Nandanwar, A. Hussain, A. Ranjan, S. Jadhav, N. Ahmed

Institutions: University of Hyderabad, Dr. D. Y. Patil Medical College, Hospital and Research Centre

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,900