Characterizing the Dynamics of the Leader–Linker Interaction in the Glycine Riboswitch with Site-Directed Spin Labeling
Year: 2014
Type: article
Abstract: Site-directed spin labeling with continuous wave electron paramagnetic resonance (EPR) spectroscopy was utilized to characterize dynamic features of the kink-turn motif formed through a leader-linker interaction in the Vibrio cholerae glycine riboswitch. Efficient incorporation of spin-labels into select sites within the phosphate backbone of the leader-linker region proceeded via splinted ligation of chemically synthesized spin-labeled oligonucleotides to in vitro transcribed larger RNA fragments. The resultant nitroxide EPR line shapes have spectral characteristics consistent with a kink-turn motif and reveal differential backbone dynamics that are modulated by the presence of magnesium, potassium, and glycine.
Source: Biochemistry
DOI: 10.1021/bi500404b
Institutions: University of Florida, University of Central Florida
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: Upgrade to E500 X- and Q-Band CW EPR Spectrometer for Biomedical Research, G3757468539, G4095418707, Targeting oncogenic RNAs with antibodies, Elucidating Molecular Mechanisms of Drug Resistance in HIV-1 Protease, Spin Labeling Insights into Hydration Effects on Macromolecular Flexibility and Function
OpenAlex ID: W2333387896