Work

Mp70-05 Development of a Novel 3 Dimensional (3d) Testicular Organoid Model for the Study of Human Spermatogenesis and Genotoxicity in Vitro

Year: 2016

Type: article

Abstract: reduction in the number of germline stem cells in vivo.To elucidate the underlying mechanism of RHOXF2 action, we optimized conditions for culturing spermatogonia from human testicular biopsies.We successfully obtained germ cells expressing spermatogonial markers that undergo proliferation in vitro.Using RNA interference (RNAi), we depleted RHOXF2 in these spermatogonia cultures CONCLUSIONS: We provide evidence that human RHOXF2 and its putative mouse ortholog, Rhox10, serve to repress the transposition of deleterious transposable elements in male germ cells.Coupled with previous finding that RHOXF2 regulates genes critical for genome defense mechanisms, it suggests that RHOXF2 serves as a guardian of genome integrity in male germ cells

Source: The Journal of Urology

DOI: 10.1016/j.juro.2016.02.1430

Authors: Samuel Pendergraft, Hooman Sadri-Ardekani, Tanya Reid, Anthony Atala, Colin Bishop

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2334334342

FWCI:

Cites:

Cited by:

Related to: 10