Year: 2016
Type: article
Abstract: The use of bioluminescent reporters in neuroscience research continues to grow at a rapid pace as their applications and unique advantages over conventional fluorescent reporters become more appreciated. Here, we describe practical methods and principles for detecting and imaging bioluminescence from live cells and animals. We systematically tested various components of our conventional fluorescence microscope to optimize it for long-term bioluminescence imaging. High-resolution bioluminescence images from live neurons were obtained with our microscope setup, which could be continuously captured for several hours with no signs of phototoxicity. Bioluminescence from the mouse brain was also imaged noninvasively through the intact skull with a conventional luminescence imager. These methods demonstrate how bioluminescence can be routinely detected and measured from live cells and animals in a cost-effective way with common reagents and equipment.
Source: Neurophotonics
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $1,675
Awards: UNS:Optogenetic Control of Neuronal Activity With Activity-Dependent Bioluminescence, Genetically Encoded Light-Production and Light-Sensing for Neuronal Manipulation, Optogenetic Population Clamp to Study Long-term Plasticity in Vitro, Autonomous Optogenetic Inhibition of Epileptic Activity Using a Bioluminescent Li, An Autonomous Biologic Controller for Optogenetic Applications
OpenAlex ID: W2334391192