Year: 2015
Type: conference-paper
Abstract: Cardiac troponin I (cTnI) is an important cardiomyocyte-specific protein responsible for heart muscle contraction. It is released into the bloodstream subsequently to heart damage; for this reason, it is widely used as a biomarker in the early detection of myocardial injuries [1,2]. Recently a novel peptide termed myocyte targeting peptide (MTP, Figure 1), which has unusual affinity to cardiomyocytes was identified by us [3,4]. This prototype MTP contains three residues of the non-natural amino acid biphenylalanine (Bip) and its selectivity towards cardiac tissues is thought to be due to the ability of Bip to bind cTnI. With the aim of early heart injury diagnosis, a library of MTP derivatives was designed in order to optimize the affinity and specificity of the troponin binding. Assisted by a fluorescein (FITC) label, the specific selectivity of the MTP analogs to myocardium over skeletal muscle tissues was assayed by fluorescence imaging. The improved MTP could potentially be used to detect circulating cTnI after cardiac injury.
Authors: Lara Pes, Young Kim, Ching-Hsuan Tung
Institutions: Weill Cornell Medicine, Chonnam National University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W2334482231