Year: 2011
Type: article
Abstract: We described in the past a number of crystal structures of enzymes in which the intermediate catalytic states have been successfully trapped and visualized.In this presentation we will describe the kinetic and thermodynamic methods of trapping the intermediates and illustrate them with four examples of recently obtained structures.Two of these examples illustrate the kinetic trapping while the other two can be described as thermodynamic enrichment of the intermediate state.We will discuss the influence of external conditions on the course of the reaction as well as specific insights reached in the individual systems.Finally, we will sketch out general lessons regarding our understanding of bio-catalysis.
Authors: B. Stec, M. F. Roberts, E. R. Kantrowitz
Language: English
Subfield: Materials Chemistry
Field: Materials Science
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2334925788