Year: 2016
Type: article
Abstract: The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully leveraged by accessing primary B-cell populations from evolutionarily divergent species such as chickens.
Source: Microscopy
Authors: Shelley Mettler Izquierdo, Stephanie Varela, Minha Park, Ellen J. Collarini, Daniel Lu, Shreya Pramanick, Joseph Rucker, Lucia Lopalco, Rob Etches, William Harriman
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,494
OpenAlex ID: W2337675328