Work

Synthesis of a peptide-universal nucleotide antigen: towards next-generation antibodies to detect topoisomerase I-DNA covalent complexes

Year: 2016

Type: article

Abstract: The topoisomerase (topo) I-DNA covalent complex represents an attractive target for developing diagnostic antibodies to measure responsiveness to drugs. We report a new antigen, peptide , and four murine monoclonal antibodies raised against that exhibit excellent specificity for recognition of in comparison to structurally similar peptides by enzyme-linked immunosorbent assays. Although topo I-DNA complex detection was not achieved in cellular samples by these new antibodies, a new strategy for antigen design is reported.

Source: Organic & Biomolecular Chemistry

DOI: 10.1039/c5ob02049b

Authors: Angela L. Perkins, Kevin L. Peterson, Thomas G. Beito, Karen S. Flatten, Scott H. Kaufmann, Daniel A. Harki

Institutions: University of Minnesota, Mayo Clinic, Mayo Clinic Comprehensive Cancer Center (Minnesota)

Language: English

Topic: Cancer therapeutics and mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: University of Minnesota, National Institutes of Health, National Cancer Institute

Awards: G1345980661, G2050332514, Cancer Center Support Grant