Year: 2014
Type: article
Abstract: Cardiotonic steroids (CTS), including ouabain and marinobufagenin, have a natriuretic effect through their action on the Na + /K + -ATPase in the kidney.In addition to a role in inducing salt-sensitive hypertension, they are also implicated in insulin regulation.CarcinoEmbryonic Antigen Cell Adhesion Molecule (CEACAM) protein, a known mediator of insulin clearance via its interaction with the insulin receptor (IR), among its other functions, is also expressed in the kidney, and involved in the development of insulin resistance.We investigate a possible interaction between cardiotonic steroids and insulin in signaling through the sodium pump toward the development of insulin resistance and salt-sensitive hypertension.The sodium pump, insulin receptor, epidermal growth factor receptor (EGFR), and CEACAM1 are expressed on the basolateral aspect of polarize renal proximal tubule LLC-PK1 cells, where they can interact to facilitate intracellular signaling.Cardiotonic steroids and insulin, both of which can signal through the Na + /K + -ATPase, stimulated decreased plasmalemmal expression, with corresponding increase in early endosomal accumulation, of CEACAM1 protein.EGFR and IR showed similar changes with exogenous ouabain or K. Malhotra for graciously accepting the mentorship role following Dr.
Author: Shalini Gupta
Institution: University of Toledo
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2338218854