Development of particulate-based EPR oximetry for regional, temporal, and rapid measurements in tissue.
Year: 2008
Type: article
Abstract: Electron paramagnetic resonance (EPR) oximetry is a useful research techniqueand a potential clinical tool.The goal of this dissertation was to establish particulate-based EPR oximetry for dynamic pO 2 measurements in vivo and widen its applications for cellular and tissue systems.EPR oximetry was compared to a fluorescence technique in an experimental tumor model.The study found EPR oximetry to be a noninvasive, robust and reliable method for repeated measurements in tumors.In addition, the response time of particulate EPR probes was established to be faster than 3.3 ms.Consequently, EPR oximetry was used for measuring temporal oxygen changes in tissue.The study revealed the presence of inherent fluctuations of oxygen in normal and tumor tissue.The frequency of fluctuations was unaltered even if modifiers of oxygen change were used.Lastly, regional pO 2 data in mouse and rat hearts were analyzed.A post-processing algorithm was used to determine components in EPR spectra obtained from probe implanted in the infarct heart.iii Overall, the above developments will help widen the scope of applications for particulate-based EPR oximetry and will further encourage evaluation of the technique as a potential clinical tool for tissue oxygen measurements.I would like to thank my advisor Dr. Periannan Kuppusamy for giving me the opportunity to work in the EPR lab on a variety of projects.In charge of a big group, he always gave priority to students, giving them plenty of opportunities to learn new things.As a mentor, he made sure that we got whatever we needed for the progress of our projects.He also encouraged us to learn and explore new research areas (quantum dots, immunology) and come up with our own ideas.He always placed emphasis on timely progress of projects, so that manuscripts could be written and published.Overall, I admire him for his futuristic, optimistic and enthusiastic ideas.There never was a dearth of projects, in fact there were too many some times!At the EPR lab, I learnt how to handle multiple projects, numerous courses and long meetings simultaneously.Finally, all the meetings would have been extremely dull, if it wasn't for my mentor's penchant for humor!I would like to thank my committee members, Drs.Govindasamy Ilangovan and Bradley Clymer, for their constant encouragement, support, and guidance.Dr. Ilangovan was an integral part of the biophysics imaging seminars vi and helped create a platform for informal discussion.Dr. Clymer's ECE 706 course built an excellent introduction and background for imaging techniques, which was very useful for a number of projects.I would like to thank Dr. Thomas Clanton for being a valuable mentor.From my first day at OSU, he has always given excellent advice and always found time to address my concerns.His assignments, a good experience for biophysics students, were tough and interesting.His approachable manner and guidance has not changed and continues through emails even after he
Author: Deepti S. Vikram
Institution: The Ohio State University
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W2340158300